One-Step Reverse Transcription PCR Reagents
AFTSpin Multifunction DNA Purification Kit
The AFTSpin Multifunction DNA Purification Kit enables rapid gel recovery and PCR product purification using silica-column technology. DNA binds to the membrane in high-salt conditions, while impurities are removed via wash steps. A unique gel lysis/binding solution (yellow for easy pH monitoring) combines gel digestion and DNA binding, streamlining agarose recovery, PCR cleanup, and enzyme digestion purification. Delivers high-purity DNA with improved efficiency in a single kit.
AFTMag Quick Blood/Cell/Tissue DNA Extraction Kit (Prepackaged)
This kit is a nucleic acid extraction kit based on bio-nanomagnetic beads. The main principle involves using functional groups on the surface of the bio-magnetic beads to enrich nucleic acids from the sample lysate onto the surface of the beads. The beads are then washed to remove proteins and other impurities. Then, a magnetic separation device is used to isolate the beads, allowing for rapid separation and purification of nucleic acids. The entire process does not require the use of organic reagents such as phenol, chloroform, or β-mercaptoethanol, making it safe and non-toxic. The use of paramagnetic magnetic beads is suitable for high-throughput automated extraction. The extracted product can be directly used for RT-PCR, RT-qPCR, Northern Blot, next-generation sequencing library construction, and cloning.
AFTSpin EndoFree Plasmid Mini Kit
This kit uses a modified SDS-alkaline lysis method to lyse cells. Crude extract is selectively bound to the spin column by a unique endotoxin scavenger and centrifuged to remove endotoxin. The silicone matrix membrane in the spin column selectively binds plasmid DNA at high salt and low pH conditions, allowing for impurities and other bacterial components to be removed by Buffer PR and Buffer WB. The purified plasmid DNA is eluted from the silicone matrix membrane using low-salt and high-pH Buffer EB.
Sigma Aldrich Fine Chemicals Biosciences Transcriptor Reverse Trans
Transcriptor Reverse Transcriptase Instructions for Use
AFTMag Quick Blood/Cell/Tissue DNA Extraction Kit
This kit is a nucleic acid extraction kit based on magnetic beads. The main principle involves using functional groups on the surface of the magnetic beads to enrich nucleic acids from the sample lysate onto the surface of the beads. The beads are then washed to remove proteins and other impurities. Then, a magnetic separation device is used to isolate the beads, allowing for rapid separation and purification of nucleic acids. The entire process does not require the use of organic reagents such as phenol, chloroform, or β-mercaptoethanol, making it safe and non-toxic. The use of paramagnetic magnetic beads is suitable for high-throughput automated extraction. The extracted product can be directly used for RT-PCR, RT-qPCR, Northern Blot, next-generation sequencing library construction, and cloning.
Genscript Corporation High-Stability PCR Kit
GenScript High-Stability PCR Kit contains Green i Taq /i DNA polymerase 10 Taq buffer and Stabilized dNTP Mix 10 mM Each all specially formulated for increased stability using our proprietary technology The kit can be stored at 4 for over six months or at room temperature (25 ) for one month without significant loss of activity The stabilizers in the buffers also increase the stability of Green Taq DNA polymerase at higher temperature (72 ) increasing the DNA yield of PCR amplifications of long DNA This kit facilitates quick and easy PCR setup without any need for thawing This kit can also be used in high-throughput assays in which all the PCR reagents need to be ready and stable for PCR setup at any time
AFTSpin Blood/Tissue/Cell Fast DNA Extraction Kit
This kit is suitable for quick and simple extraction of genomic DNA from various samples inclouding blood, cells and tissue. The extracted genomic DNA fragment are large, high purity and good stability, and can be directly used in PCR, enzyme digestion and hybridization experiments. The extraction process does not require phenol-chloroform extraction. Blood, cells or tissues are lysed by lysate and digested by Proteinase K, and then can be combined with Spin Column 4 under the regulation of anhydrous ethanol. After quickly and sufficiently washing, residual impurities such as protein and salt are removed, and finally DNA is dissolved in Buffer EB2.
TruePol 2X PCR Mix for NGS
TruePol 2X PCR Mix for NGS is a premixed solution derived from polymerase of the B family. It is easy to use, containing DNA polymerase, buffer, Mg2+, and dNTPs. For amplification, simply add DNA template, primers, and water to the system for amplification. This polymerase comes from a unique polymerase mutation library, offering strong extension capabilities that improve yield and specificity without significant GC bias. It has 5-3 continuous synthesis activity and 3-5 exonuclease activity, resulting in blunt-end amplification products. All batches of TruePol Mix undergo rigorous quality control to ensure the consistent performance of each batch of reagent.
Alkali Scientific Radiant™ Probe 1-Step Lo-Rox qPCR Virus Kit, 1000 x 20µl Reactions, 4X
Recommended kit for SARS-CoV-2 diagnostics Validated for qualitative detection of SARS-CoV-2 nucleic acid Concentrated 4x mix format, ideal for high-throughput, highly multiplexed assays Ultra-sensitive detection of RNA viruses Earlier detection of a wide range of template amounts Includes UltraScript RTase for thermostable reverse transcription up to 55°C Advanced RNase inhibitor Antibody-mediated hot start technology Compatible with all real-time PCR platforms – standard and fast cycling conditions The kit has been optimised with a high-concentration 4x mix, enabling greater sample input and increasing sensitivity, even when working with small volume reactions.
Lyophilization One Step RT-qPCR Probe Kit
The Lyophilization One-Step RT-qPCR Probe Kit is a ready-to-use, freeze-dried reagent for probe-based RT-qPCR, compatible with lyophilized powder or microspheres. It integrates ABScript III Reverse Transcriptase, hot-start Taq polymerase, heat-labile UDG, and RNase inhibitor for efficient one-tube reactions, boosting throughput. The dUTP/UDG system prevents contamination, with UDG degrading at 50°C to avoid interference. Stable at 45°C for 6 months, it contains <0.2% glycerol and lyoprotectants for long-term performance.